Fabrication Solid-State Nanopores
The main strength of nanopore sensing is that it implies the prospect of label-free single-molecule detection by taking advantage of the built-in transport-modulation-based amplification mechanism. We were presented a line of nanopore based sensor development, including methodologies for fabrication and functional characterization of solid state nanopores, their chemical modification and application for bio(chemical) sensing.
We described a reliable nanofabrication technology of solid state nanopore arrays. The geometric parameters of pores are achieved and optimized according to the requirements of bioanalytical applications regarding conformation and size of the characteristic proteins in clinical diagnostics. Different structural configurations and material compositions of the nanopore structures were developed and characterised according to the variable biofunctionalisation strategies. The geometry of the fabricated pores were analysed as the statistical function of the focused ion beam milling parameters.
The bioanalytical systems are expected to integrate the nanoscale transducers with interface chemistry, and bio-receptors as well as the microfluidics, control software and hardware.
Solid-state nanopore arrays were integrated into complex microfluidic environment enabling both fluidic and electrical addressing and their applicability was proved as biosensor by detection avidin-biotin and also human immunoglobulin-E (hIgE) specific aptamer and hIgE binding process using electrochemical impedance spectroscopy (EIS).
The micro- and nanotechnology based biosensing principles open up new possibilities towards the development and realisation of robust, user-friendly and cost-effective in-vitro diagnostic platforms. Furthermore, label-free and multi- analyte detection is envisioned to allow more accuracy and higher throughput in clinical diagnostic. The final goal of this work is to develop nanopore based multi-parametric biosensing platform, applicable for label-free detection of marker molecules.